Immortalization of normal human kidney epithelial cells by nickel(II).

Altered p53 gene structure and expression in human epithelial cells after exposure to nickel.

The carcinogenicity of certain nickel compounds is well known. We have previously shown that human kidney epithelial cells were immortalized by treatment with Ni(II) and in cooperation with the v-Ha-ras oncogene transformed the cells to acquire tumorigenicity in athymic nude mice. Immunocytochemistry and sequence analysis of DNA from the nickel-immortalized cells revealed abnormal p53 expressio...

Nickel-induced alterations in human renal epithelial cells.

Cellular progression to malignancy appears to require a number of distinct steps in which genetic damage in key regulatory genes accumulates. Immortalization, or escape from senescence, is considered to be one of the first phenotypic changes. Ni2+ treatment of normal human kidney epithelial (NHKE) cells in vitro resulted in immortalization of the cells IHKE cells). The combined action of Ni2+ a...

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro

Apoptosis, bcl2 expression, and cell cycle analyses in nickel(II)-treated normal rat kidney cells.

Nickel compounds are carcinogenic to human and are potent inducers of kidney and lung tumors in experimental animals. In this study, the effects of nickel(II) acetate on apoptosis, cell cycle and bcl2 expression in normal rat kidney (NRK- 52E) cells were investigated. Nickel(II) induced apoptosis in NRK-52E cells as demonstrated by DNA laddering. Apparent DNA laddering was observed in cells tre...

Evaluation of silica nanoparticles cytotoxicity (20-40 nm) on cancerous epithelial cell (A549) and fibroblasts cells of human normal lung fibroblast (MRC5)

Introduction: Silica nanoparticles have received more attraction in medical and industrial applications due to their unique properties such as small size, the possibility of surface functionalization, ease of production, and low cost. So, it is necessary to study the respiratory toxicity of occupational exposure due to the production and increasing use of silica nanoparticles, especially in the...